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KMID : 0604520120380010075
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Journal of the Society of Cosmetic Scientists of Korea
2012 Volume.38 No. 1 p.75 ~ p.82
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Study on the Relationship between Skin Dryness and Amino Acids in Stratum Corneum
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Joo Kyeong-Mi
Han Ji-Yeon
Son Eui-Dong
Nam Kae-Won
Jung Hye-Jin
Lim Kyung-Min
Cho Jun-Cheol
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Abstract
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Natural moisturizing factors (NMFs) are hydrophilic and water-soluble components in stratum corneum of the skin. NMFs absorb water from outer environment and enhance the water-holding capacity of stratum corneum and thereby, prevent the dryness and increase flexibility and plasticity of skin. NMFs are mainly composed of amino acids and their metabolites that are produced from the degradation of filaggrin. Here we established a simultan eous quantification method for 22 amino acids in tape-stripped stratum corneum samples using UPLC-PDA. With this method, we analyzed amino acid contents from tape-stripped stratum corneum samples of forearm and forehead regions from 15 healthy volunteers. Amino acid contents of inner (or upper) region were higher than outer (or lower) region of stratum corneum. Amino acid contents of stratum corneum of forearm were higher by 1.5 fold than forehead region. Of note, total amino acid contents were significantly and inversely correlated with trans-epidermal water loss (TEWL), an index for skin barrier function. Especially, Ser, Glu, Gly, Ala and Thr were determined to positively affect skin mositurizing activities. In conclusion, we could demonstrate that amino acid contents of stratum corneum are closely linked with skin barrier and moisturizing function, providing an important and fundamental methodology for the study of amino acids in skin physiology.
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KEYWORD
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amino acids, human stratum corneum, natural moisturizing factor (NMF), UPLC-PDA
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